Sensitive and rapid detection of Shigella and enteroinvasive Escherichia coli by a loop-mediated isothermal amplification method |
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| Authors: | Song Tianyan Toma Claudia Nakasone Noboru Iwanaga Masaaki |
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| Institution: | Division of Bacterial Pathogenesis, Department of Microbiology, Graduate School of Medicine, University of the Ryukyus, 207 Uehara, Nishihara, Okinawa 903-0215, Japan. k018763@med.u-ryukyu.ac.jp |
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| Abstract: | Here we report a loop-mediated isothermal amplification (LAMP) method for detecting Shigella and enteroinvasive Escherichia coli. The target for this LAMP method is the ipaH gene which is carried by both of the pathogens. The LAMP method efficiently detected the gene within 2 h at a minimal amount of bacteria (8 CFU) per reaction. |
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| Keywords: | Shigella Enteroinvasive Escherichia coli LAMP ipaH gene detection |
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