The Flp double cross system a simple efficient procedure for cloning DNA fragments |
| |
Authors: | Email author" target="_blank">Paul?D?SadowskiEmail author |
| |
Institution: | (1) Department of Medical Genetics and Microbiology, University of Toronto, Toronto, M5S 1A8, Canada |
| |
Abstract: | Background While conventional cloning methods using restriction enzymes and polynucleotide ligase are adequate for most DNAs, fragments
made by the polymerase chain reaction are difficult to clone because the amplifying DNA polymerase tends to add untemplated
nucleotides to the 3'-termini of the amplified strands. Conservative site-specific recombinases offer an efficient alternative
to conventional cloning methods. |
| |
Keywords: | |
本文献已被 SpringerLink 等数据库收录! |