Cloning of a vacuolar invertase from Belgian endive leaves (Cichorium intybus) |
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Authors: | Van Den Ende Wim Michiels An Le Roy Katrien Van Laere André |
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Institution: | Department of Biology, Laboratory for Developmental Biology, Botany Institute, KULeuven, Kasteelpark Arenberg 31, B-3001 Leuven, Belgium |
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Abstract: | Although a lot of vacuolar invertase (EC 3.2.1.26) cDNAs are available from a diversity of plant species, up to now no sequence information is available on invertases from any dicot fructan-containing species. Therefore, we describe the cloning of vacuolar acid invertase cDNA from etiolated Belgian endive leaves ( Cichorium intybus L. var. foliosum cv. Flash), formed throughout the forcing process of the witloof chicory roots. Full-length cDNA was obtained by a combination of RT-PCR, PCR and 5'- and 3' RACE RT-PCR, starting with primers based on conserved amino acid sequences. The cloned chicory acid invertase groups together with vacuolar type invertases and fructan biosynthetic enzymes. A putative role for vacuolar type invertases in fructan synthesizing plants is discussed. |
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