RNA microarray for estimating relative abundance of 16S rRNA in microbial communities |
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| Authors: | Hoshino Tatsuhiko Furukawa Kazuhiro Tsuneda Satoshi Inamori Yuhei |
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| Institution: | Department of Chemical Engineering, Waseda University, 3-4-1 Ohkubo, Shinjuku-ku, Tokyo 169-8555, Japan. hoshino@microbial-ecology.net |
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| Abstract: | We developed an RNA microarray protocol in which total RNA from a microbial community was attached to a slide glass, and rRNA was detected by fluorescently labeled oligonucleotide probes. The RNA microarray requires only 4 h for hybridization and enables double staining and estimating relative abundance of rRNA. |
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