Molecular recognition of nucleic acids: coralyne binds strongly to poly(A) |
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| Authors: | Xing Feifei Song Guangtao Ren Jinsong Chaires Jonathan B Qu Xiaogang |
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| Affiliation: | Key Laboratory of Rare Earth Chemistry and Physics, Changchun Institute of Applied Chemistry, Graduate School of the Chinese Academy of Sciences, Chinese Academy of Sciences, Changchun, Jilin 130022, China. |
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| Abstract: | The small molecule coralyne was found to bind preferentially and strongly to single-stranded poly(A) with an apparent association constant (Ka) of (1.8+/-0.3) x 10(6)M(-1). Binding of coralyne to poly(A) is predominantly enthalpically driven with a stoichiometry of one coralyne per four adenine bases. Poly(A) forms a coralyne dependent secondary structure with a melting temperature of 60 degrees C, for the conditions of our study. |
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| Keywords: | DNA RNA Dialysis Binding Single-stranded poly(A) Secondary structure |
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