Geobacillus uralicus,a New Species of Thermophilic Bacteria |
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Authors: | Popova N A Nikolaev Yu A Tourova T P Lysenko A M Osipov G A Verkhovtseva N V Panikov N S |
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Institution: | (1) Institute of Microbiology, Russian Academy of Sciences, pr. 60-letiya Oktyabrya 7, k. 2, Moscow, 117312, Russia;(2) Yaroslavl State University, Russia;(3) Research Institute for Cardio-Vascular Surgery, Russian Academy of Medical Sciences, Moscow;(4) Soil Science Faculty, Moscow State University, Vorob'evy gory, Moscow, 119899, Russia |
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Abstract: | The KT
2 strain of thermophilic spore-forming bacteria was isolated from a biofilm on the surface of a corroded pipeline in an extremely deep well (4680 m, 40–72°C) in the Urals. The cells are rod-shaped, motile, gram-variable. They grow on a complex medium with tryptone and yeast extract and on a synthetic medium with glucose and mineral salts without additional growth factors. The cells use a wide range of organic substances as carbon and energy sources. They exhibit a respiratory metabolism but are also capable of anaerobic growth on a nitrate-containing medium. Growth occurs within the 40–75°C temperature range (with an optimum of 65°C) and at pH 5–9. The minimum generation time (15 min) was observed at pH 7.5. Ammonium salts, nitrates, and arginine are used as nitrogen sources. The G+C content of the DNA is 54.5 mol %. From the morphological, physiological, and biochemical properties and the nucleotide sequence of the 16S rRNA gene, it was concluded that the isolate KT
2 represents a new species of the genus Geobacillus, Geobacillus uralicus. |
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Keywords: | thermophilic bacteria Geobacillus uralicus taxonomy |
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