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Identification of an operon involved in the assimilatory nitrate-reducing system of Azotobacter vineiandii
Authors:Francisco Ramos  Gonzalo Blanco  Juan C Gutiérrez  Francisco Luque  Maria Tortolero
Institution:Departamento de Mlcrobiologia, Pacultad de Biologia, Universidad de Sevilla, Apartado 1095. E-41060 Seviila, Spain.;Departamento de Genética, Facultad de Biologia, Universidad de Sevilla, Apartado 1095, E-41080 Sevilla, Spain.
Abstract:A number of mutants lacking nitrate reductase (Nas?) or nitrite reductase (Nis?) activities have been isolated and characterized. An operon including two new genes (nasA and nasB) has been defined and cloned from an Azotobacter vinelandii gene bank. nasA encodes for nitrite reductase apoenzyme, whereas nasB is specific for nitrate reductase activity. Nitrate reductase exerts a regulatory effect on nasAB.
Keywords:
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