The metamorphic switch in hemoglobin phenotype ofXenopus laevis involves erythroid cell replacement |
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Authors: | Rudolf Weber Marianne Geiser Peter Müller Erika Sandmeier Toni Wyler |
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Institution: | (1) Abteilung für Zell-und Entwicklungsbiologie, Zoologisches Institut, Baltzerstrasse 4, CH-3012 Bern, Switzerland |
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Abstract: | Summary To elucidate the cellular basis of hemoglobin transition inXenopus laevis the distribution of larval and adult hemoglobins was analyzed by indirect immunofluorescence in the circulating erythrocytes
during metamorphosis. In addition, the morphological characteristics as well as the capacity for synthesis of DNA and hemoglobin
in the erythrocytes were followed during the same developmental period. Our quantitative analysis on the distribution of larval
and adult hemoglobins suggests that they are localized in different cells. Hemoglobin transition, therefore, most likely reflects
replacement of the larval erythrocyte population by new cells which are committed to adult globin synthesis. Since hemoglobin
transition is not accompanied by an increase in the abundance of immature erythroid cells with active DNA synthesis, we assume
that the presumptive adult erythroid cells are released into circulation at a relatively advanced stage of maturation. The
decline in the synthesis of DNA and larval hemoglobin further indicates that cessation of cell renewal in the larval erythrocyte
population may represent a decisive step in hemoglobin transition. |
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Keywords: | Hemoglobin transition Erythrocytes Indirect immunofluorescence Metamorphosis Xenopus laevis |
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