The antitumor activity of the hydrolysates of chitinous materials hydrolyzed by crude enzyme from Bacillus amyloliquefaciens V656 |
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| Institution: | 1. Department of Bioindustry Technology, Da-Yeh University, Chanhwa 515, Taiwan;2. Department of Radiation Oncology, Mackay Memorial Hospital, Taipei 104, Taiwan;3. Graduate Institute of Life Sciences, Tamkang University, Tamsui 251, Taiwan;4. Life Science Development Center, Tamkang University, Tamsui 251, Taiwan;1. Department of Marine Biology, Faculty of Marine Sciences and Technology, University of Hormozgan, Bandar Abbas, Iran;2. Department of Polymer Engineering, Graduate University of Advanced Technology, Kerman, Iran;3. Department of Microbiology, Fasa University of Medical Sciences, Fasa, Iran;1. Department of Environmental and Food Sciences, Fukui University of Technology, Fukui 910-8505, Japan;2. Institue for Protein Research, Osaka University, Suita 565-0871, Japan;3. Department of Advanced Bioscience, Kinki University, Nara 631-8505, Japan;4. Department of Bioscience, Fukui Prefectural University, Eiheiji-cho, Yoshida-gun, Fukui 910-1195, Japan;1. Universidad Autónoma Metropolitana, Biotechnology Department, Laboratory of Biopolymers and Pilot Plant of Bioprocessing of Agro-Industrial and Food By-Products, Av. San Rafael Atlixco No. 186, Col. Vicentina, C.P. 09340, Iztapalapa, México City, Mexico;2. Universidad Nacional Autónoma de México, Facultad de Química, Ciudad Universitaria, C.P. 04510, Mexico City, Mexico;3. Ingénierie des Matériaux Polymères IMP@Lyon1, UMR CNRS 5223, Université Claude Bernard Lyon 1, Université de Lyon, 15 bd A. Latarjet, Villeurbanne Cedex, France;4. Graduate School of Engineering, Department of Chemistry and Biotechnology, Tottori University, Koyamacho-minami 4-101, Tottori City, Tottori Prefecture 680-8550, Japan;1. Universidad Autonoma Metropolitana, Departamento de Biotecnologia, Laboratorio de Biopolimeros, San Rafael Atlixco 186, Mexico City C.P. 09340, Mexico;2. Universidad Nacional Autonoma de Mexico, Facultad de Quimica, Mexico City C.P. 04510, Mexico;1. School of Biotechnology, State Key Laboratory of Bioreactor Engineering, R&D Center of Separation and Extraction Technology in Fermentation Industry, East China University of Science and Technology, Shanghai 200237, China;2. Shanghai Collaborative Innovation Center for Biomanufacturing Technology (SCICBT), Shanghai 200237, China |
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| Abstract: | Chitin, colloidal chitin and water-soluble chitosan were hydrolyzed by crude enzyme solution produce by Bacillus amyloliquefaciens V656. The hydrolysates with 12 h hydrolysis contained optimal (GlcNAc)6 and showed higher antitumor activity. Among those chitinous materials, the most effective one was the hydrolysates of water-soluble chitosan, which inhibited the growth of CT26 cells and reduced the survival rate to 34% in 1 day. Since the hydrolysate of water-soluble chitosan contained the optimal hexamer/(GlcNAc)6 at 12 h, it is conjectured that the antitumor activity should be related to (GlcNAc)6. This conjecture was further affirmed by experiment with pure (GlcNAc)6. However, This phenomenon might be due to the synergistic effect of the oligomers (GlcNAc)n, n = 1–6 in the hydrolysates. The antitumor effect of the chitinous hydrolysates is worth further investigation.The aim of this study was to investigate the induced apoptosis in CT26 cells by the hydrolysates of chitinous materials. It was found that the hydrolysates (A, B and C) inhibited the survival of CT26 cells in a concentration- and time-dependent manner. The hydrolysates induced characteristic DNA fragmentation of the CT26 cells. These results suggested that the hydrolysates from chitinous materials are potent apoptosis-inducing agents for CT26 cells. |
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