Regeneration of denatured polyphenol oxidase in Toona sinensis (A.Juss.) Roam |
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| Institution: | 1. College of Food Science and Nutritional Engineering, China Agricultural University, P.O. Box 111, Qinghua Donglu No. 17, Beijing 100083, China;2. Light Industry and Food College, Zhongkai University of Agriculture and Technology, Fangzhi Road, Dongsha Street No. 24, Guangzhou 510225, China;1. Instituto de Tecnología de Alimentos, Facultad de Ingeniería Química, Universidad Nacional del Litoral, Santiago del Estero 2829, Santa Fe 3000, Argentina;2. Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Santiago del Estero 2829, Santa Fe, Argentina;3. Plants for Human Health Institute, Department of Food, Bioprocessing and Nutrition Sciences, North Carolina State University, 600 Laureate Way, Kannapolis, NC 28081, United States;1. Department of Veterinary Clinics, University of Pisa, San Piero a Grado Pisa, Italy;2. Veterinary Clinic Roma Sud, Roma, Italy;3. CVS Centro Veterinario Specialistico, Perugia, Italy;4. Department of Pathology and Animal Health, Faculty of Veterinary Medicine, University of Naples Federico II, Naples, Italy;1. Jiangxi Key Laboratory of Natural Product and Functional Food, College of Food Science and Engineering, Jiangxi Agricultural University, Nanchang 330045, China;2. Academic Affairs Division, Jiangxi Agricultural University, Nanchang 330045, China;1. Sección Departamental de Tecnología de los Alimentos, Facultad de Veterinaria, Universidad Complutense de Madrid, 28040 Madrid, Spain;2. CAI de Resonancia Magnética y de Espín Electrónico, Universidad Complutense de Madrid, 28040 Madrid, Spain;1. Department of Nutrition, School of Public Health, Iran University of Medical Sciences, Tehran, Iran;2. Nanobiotechnology Research Center, Avicenna Research Institute, ACECR, Tehran, Iran Immunology Research Center, Iran University of Medical Sciences, Tehran, Iran;3. Immunology Research Center, Iran University of Medical Sciences, Tehran, Iran;4. National Institute and Faculty of Nutrition and Food Technology, Department of Basic Medical Sciences, Shahid Beheshti University of Medical Sciences, Tehran, Iran;5. Department of Cellular Molecular Nutrition, School of Nutritional Sciences and Dietetics, Tehran University of Medical Sciences, Tehran, Iran;6. Monoclonal Antibody Research Center, Avicenna Research Institute, ACECR, Tehran, Iran;1. School of Energy Science and Engineering, Central South University, Changsha, 410083, China;2. Engineering College, Guangdong Ocean University, Zhanjiang, 524088, China |
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| Abstract: | The regeneration of polyphenol oxidase (PPO) from leaves of Toona sinensis (A.Juss.) Roam (TS), denatured by boiling with 0.4% (w/v) sodium dodecyl sulfate (SDS) and 1% (v/v) β-mercaptoethanol, and was studied with SDS-polyacrylamide gel electrophoresis (PAGE) and the gel-activity-stained assay. The optimal regenerating conditions for the denatured TS-PPO were as follows: extracting with buffer of 150 mM Na-Pi, pH 7.2; regenerating with buffer of 50 mM Tris–HCl, pH 7.2, 2% Triton X-100 for 45 min. In addition, the PPO regeneration was promoted by the addition of Cu2+ in the recovery buffer. The regenerating activity of the denatured-PPO was reduced in samples extracted with buffers containing polyvinylpyrrolidone (PVP), polyvinylpolypyrrolidone (PVPP) or Triton X-100. |
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