Isolation and identification of a novel Rhodococcus sp. ML-0004 producing epoxide hydrolase and optimization of enzyme production |
| |
| Institution: | 1. Institute of Bioengineering, Zhejiang University of Technology, Hangzhou 310014, PR China;2. Department of Plant Science, North Dakota State University, Fargo 58105, USA;3. Institute of Quality and Standard for Agricultural Products, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, PR China;4. School of Food Engineering & Biotechnology, Jiangsu University, Zhenjiang 2120013, PR China;1. Laboratorio de Biocatálisis y Biotransformaciones, Universidad Nacional de Quilmes, Bernal 1876, Argentina;2. Instituto de Química Rosario (IQUIR, CONICET-UNR, Unidad Orgánica) y Facultad de Ciencias Bioquímicas y Farmacéuticas, Universidad Nacional de Rosario, Rosario S2002LRK, Argentina;3. Laboratorio de Micología Molecular, Universidad Nacional de Quilmes, Bernal 1876, Argentina;1. Department of Food Science and Human Nutrition, Iowa State University, Ames, IA, United States;2. Department of Food Science, Cornell University, Ithaca, NY, United States;1. Division of Biomedical Convergence, College of Biomedical Science, Kangwon National University, Chuncheon 24341, Republic of Korea;2. Department of Molecular Bioscience, School of Biomedical Science, Kangwon National University, Chuncheon 24341, Republic of Korea;3. College of Veterinary Medicine, Kangwon National University, Chuncheon 24341, Republic of Korea;4. Laboratory of Microbiology and Immunology, College of Pharmacy, Kangwon National University, Chuncheon 24341, Republic of Korea;5. Institute of Bioscience and Biotechnology, Kangwon National University, Chuncheon 24341, Republic of Korea;1. Beijing Advanced Innovation Center for Food Nutrition and Human Health, Beijing Technology and Business University (BTBU), Beijing, 100048, People''s Republic of China;2. Beijing Engineering and Technology Research Center of Food Additives, Beijing Technology and Business University (BTBU), Beijing, 100048, People''s Republic of China;1. Faculty of Conservation and Restoration of Works of Art, Jan Matejko Academy of Fine Arts in Kraków, Poland;2. Małopolska Centre of Biotechnology, Jagiellonian University, Kraków, Poland;3. Institute of Environmental Sciences, Jagiellonian University, Kraków, Poland |
| |
| Abstract: | Rhodococcus sp. ML-0004, a novel strain for producing epoxide hydrolase, was isolated from soil in this study. The epoxide hydrolase can catalyze the stereo-specific hydrolysis of cis-epoxysuccinic acid to generate l(+)-tartaric acid. By examining physiological, biochemical characteristics and comparing its 16S rDNA gene sequence, it was identified as Rhodococcus opacus, and named R. opacus ML-0004. The optimal conditions for epoxide hydrolase production from R. opacus ML-0004 were also investigated. Propanediol and (NH4)2SO4 were selected as carbon source and nitrogen source, respectively, for the production of R. opacus ML-0004 epoxide hydrolase. The optimal conditions for epoxide hydrolase production were fermentation temperature = 28 °C, pH 7.0, and cultivation time = 26 h. Under these conditions, the maximum epoxide hydrolase activity reached 10.5 U mL−1. |
| |
| Keywords: | |
| 本文献已被 ScienceDirect 等数据库收录！ |
|
