Ram horn hydrolysate as enhancer of xanthan production in batch culture of Xanthomonas campestris EBK-4 isolate |
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| Institution: | 1. Center of Biophotonics, School of Dentistry, Federal University of Bahia – UFBA, Av. Araújo Pinho, 62, Canela, Salvador, BA CEP: 40110-150, Brazil;2. Laboratory of Biotechnology and Ecology of Microorganisms, Institute of Health Science, Federal University of Bahia, Reitor Miguel Calmon Ave, S/N, Salvador, BA CEP: 40110-100, Brazil;3. NextGen DNA Sequencing Scientific Director, UF-ICBR, Bldg CGRC, Room 178, 2033 Mowry Road, Gainesville, FL 32610, United States;4. University of St Andrews UK, School of Physics & Astronomy, Physical Science Building, North Haugh, St Andrews, Fife KY16 9SS, United Kingdom;5. Center for Innovation, Technology and Education – CITE, Universidade Anhembi Morumbi – UAM, Estr. Dr. Altino Bondensan, 500, São José dos Campos, SP CEP: 12247-016, Brazil |
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| Abstract: | Ram horns are waste materials from the meat industry. The objective of this study was to investigate the effects of various concentrations of ram horn (RHH) hydrolysate as a supplement on xanthan production from a local isolate of Xanthomonas campestris EBK-4 in batch culture. Firstly, ram horn hydrolysate was reproduced. The production of xanthan was influenced by the RHH. RHH supplementation promoted X. campestris growth, accelerated substrate metabolism, and increased xanthan production. A concentration of 3% v/v RHH resulted in the highest xanthan concentration (25.6 g/L) in 48 h. This value was 49% higher than that of control medium (17.1 g/L) in the absence of RHH in 60 h. The pyruvate content increased with increasing RHH concentrations. The application of RHH resulted in enhancement of xanthan production. |
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