Optimization of free radical scavenging activity by response surface methodology in the hydrolysis of shrimp processing discards |
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| Affiliation: | 1. Université de Bretagne Occidentale, Laboratoire ANTiOX, Pôle Universitaire P.J. Helias, Creac’h Gwen, F-29000 Quimper, France;2. Université de La Réunion, Laboratoire de Chimie des Substances Naturelles et des Sciences des Aliments, 15 Avenue René Cassin, BP 7151, F-97715 Saint-Denis Messag Cedex 9, La Réunion, France;1. Department of Applied Chemistry, University of Johannesburg, Johannesburg, South Africa;2. Young Researchers and Elites Club, Science and Research Branch, Islamic Azad University, Tehran, Iran;3. Department of Chemistry, Central Tehran Branch, Islamic Azad University, Tehran, Iran;4. Department of Chemical Engineering, North Tehran Branch, Islamic Azad University, Tehran, Iran;1. Université de Caen Basse-Normandie, UMR BOREA, IBFA, F-14032 Caen, France;2. UMR BOREA Biologie des ORganismes et Ecosystèmes Aquatiques” MNHN, UPMC, UCBN, CNRS-7208, IRD-207, France;3. Proteogen, SF 4206 ICORE, Université de Caen Basse-Normandie, F-14032 Caen, France;4. Aquativ (Diana Group), ZA du Gohelis, 56250 Elven, France;5. ABiMS, FR2424 CNRS-UPMC, Station Biologique, 29680 Roscoff, France;1. Program in Genetics and Genome Biology, Hospital for Sick Children, Toronto, ON, Canada;2. Department of Immunology, University of Toronto, Toronto, ON, Canada;3. Department of Medical Biophysics, University of Toronto, Toronto, ON, Canada;1. Institute of Bioresources and Sustainable Development, Regional Centre, Gangtok, India;2. Centre of Innovative and Applied Bioprocessing, Mohali, India;3. Institute of Bioresources and Sustainable Development, Mizoram Node, Aizawl, India |
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| Abstract: | Response surface methodology (RSM) was used to optimize the hydrolysis conditions (temperature, pH and Alcalase® 2,4L concentration), in order to obtain the hydrolysate with the strongest antioxidant activity using the 1,1-diphenyl-2-picryl-hydrazyl (DPPH ) decolouration assay. The optimum conditions obtained from experiments were pH 9.7, 66.2 °C, enzyme concentration = 68.1 Anson units (AU)/kg crude protein. The analysis of variance in RSM showed that pH and temperature (T) were the most important factors of the process (P < 0.001). The experimental conditions produced both an enzymatic and chemical protein solubilization. |
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