Effect of amino acids addition and feedback control strategies on the high-cell-density cultivation of Saccharomyces cerevisiae for glutathione production |
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| Affiliation: | 1. Key laboratory of Biomass Chemical Engineering (Ministry of Education), College of Chemical and Biological Engineering, Zhejiang University, Hangzhou 310027, China;2. Xiangya International Academy of Translational Medicine, Central South University, Changsha 410013, Hunan, China;1. State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi 214122, China;2. Key laboratory of Industrial Biotechnology of Ministry of Education, School of Biotechnology, Jiangnan University, 1800 Lihu Avenue, Wuxi 214122, China;3. Synergetic Innovation Center of Food Safety and Nutrition, Jiangnan University, Wuxi 214122, China;1. Departments of Pediatrics and Genetics, Stanford University School of Medicine, Stanford, California, USA;1. Department of Chemical and Biochemical Engineering, Technical University of Denmark, 2800 Kgs. Lyngby, Denmark;2. Novozymes A/S, Krogshoejvej 36, 2880 Bagsvaerd, Denmark |
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| Abstract: | Controlling glucose feeding rate, according to the change of ethanol concentration and respiratory quotient (RQ), has been studied in high-cell-density cultivation of Saccharomyces cerevisiae for glutathione (GSH). GSH yield and dry cell weight reached 1620 mg/L and 140 g/L, respectively, after 52 h of cultivation. In addition, optimized single shot addition of precursor amino acids (performed in both flask and fermentor experiments) at 32 h could result in the GSH yield reached 2020 mg/L after 38 h of cultivation. The yield and productivity of GSH increased 25% and 70%, respectively. Moreover, cultivation time was reduced to 38 h, compared with results without adding the precursor amino acids. |
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