Development of continuous chitinase production process in a membrane bioreactor by Paenibacillus sp. CHE-N1

In this research, the feasibility of using membrane mode fermentation operations for the continuous chitinase production by Paenibacillus sp. CHE-N1 was investigated. The bioreactor with a membrane outer recycling loop was used to evaluate the effect of membrane pore size on cell retention efficiency, permeate flow rate, fouling, and chitinase recovery in permeate. The results showed that at a transmembrane pressure of 0.9 kg/cm², M 9 microfiltration column with a nominal pore size of 300 kDa exhibited the best microfiltration characteristics and was used for the membrane mode operation. As comparing the chitinase production in the membrane mode operation by feeding deionized water with that in batch mode, the total chitinase activity obtained in membrane operation could reach 42,800 mU for 132 h, about 78% higher than that obtained in batch mode operation. Further improvement by feeding chitin every 3–4 days showed a steadily continuous chitinase production with the activity ranging from 13 to 15 mU/ml at a flow rate of 500 ml/day. The membrane-based microfiltration operation appears to be useful for enhancing the chitinase activity production in fermentation.