Optimization of the freeze-drying media and survival throughout storage of freeze-dried Lactobacillus gasseri and Lactobacillus delbrueckii subsp. delbrueckii for veterinarian probiotic applications |
| |
| Institution: | 1. Division of Food Sciences, School of Biosciences, University of Nottingham, Sutton Bonington Campus, Loughborough LE12 5RD, Leicestershire, United Kingdom;2. Nottingham Nanotechnology and Nanoscience Centre, University of Nottingham, University Park, Nottingham NG7 2RD, Nottinghamshire, United Kingdom;1. School of Food Science and Technology, Jiangnan University, Wuxi, 214122, China;2. Guelph Research and Development Centre, Agriculture and Agri-Food Canada, Guelph, Ontario, N1G 5C9, Canada;3. Ottawa Research and Development Centre, Agriculture and Agri-Food Canada, Ottawa, Ontario, K1A 0C6, Canada;1. Riddet Institute, Massey University, Palmerston North, New Zealand;2. Institute of Fundamental Sciences, Massey University, Palmerston North, New Zealand;3. School of Engineering and Advanced Technology, Massey University, Palmerston North, New Zealand;4. Institute of Food, Nutrition and Human Health, Massey University, Palmerston North, New Zealand;1. Department of Food Science and Technology, Sabzevar Branch, Islamic Azad University, Sabzevar, Iran;2. Department of Food Science and Technology, School of Agriculture, Shiraz University, Shiraz, Iran;3. Department of Food Science and Technology, Ferdowsi University of Mashhad, Mashhad, Iran |
| |
| Abstract: | The use of lactic acid bacteria (LAB) as vaginal probiotic cultures depends on the preservation technologies employed by the related industries.A full two-factor analysis of variance (ANOVA), considering medium and strain, of the decrease in bacterial viability during freeze-drying was applied. Lactobacillus gasseri CRL1421 was significantly more resistant than L. gasseri CRL1412 to the process. L. gasseri CRL1412 suspended in skim milk showed a significantly higher resistance than when it was suspended in water, but lactose or sucrose did not significantly increase its viability after lyophilization. Lactobacillus delbrueckii subsp. delbrueckii CRL1461, an autoaggregative strain, was significantly more sensitive to freeze-drying under the assessed conditions.The dried cultures were included in two pharmaceutical forms and viability was monitored during 270 days of storage. Although the microorganisms studied belonged to the same species, the optimal storage conditions were different for each of them.Our results can be applied to the design of a veterinarian probiotic to prevent metritis in diary postpartum cows. |
| |
| Keywords: | |
| 本文献已被 ScienceDirect 等数据库收录！ |
|
