Simultaneous determination of celecoxib,hydroxycelecoxib, and carboxycelecoxib in human plasma using gradient reversed-phase liquid chromatography with ultraviolet absorbance detection |
| |
Authors: | Störmer Elke Bauer Steffen Kirchheiner Julia Brockmöller Jürgen Roots Ivar |
| |
Affiliation: | Institute of Clinical Pharmacology, University Medical Center Charité, Humboldt University Berlin, Schumannstrasse 20/21, 10098, Berlin, Germany. elke.stoermer@charite.de |
| |
Abstract: | A new HPLC method for the simultaneous determination of celecoxib, carboxycelecoxib and hydroxycelecoxib in human plasma samples has been developed. Following a solid-phase extraction procedure, the samples were separated by gradient reversed-phase HLPC (C(18)) and quantified using UV detection at 254 nm. The method was linear over the concentration range 10-500 ng/ml. The intra-assay variability for the three analytes ranged from 4.0 to 12.6% and the inter-assay variability from 4.9 to 14.2%. The achieved limits of quantitation (LOQ) of 10 ng/ml for each analyte allowed the determination of the pharmacokinetic parameters of the analytes after administration of 100 mg celecoxib. |
| |
Keywords: | Celecoxib Hydroxycelecoxib Carboxycelecoxib |
本文献已被 ScienceDirect PubMed 等数据库收录! |