Perifusion system: its use in the study of the neuroendocrine control of human pituitary tumoral cells

Conclusion Taken together, these results show the usefulness of the perifusion technique both in the studyof hormone regulation and in the physiopathology of the human pituitary. It allows the studyof dynamic changes in hormone release, relationships between in vivo and in vitro responses, relationships between hormone response and receptor status. Furthermore, we could use this approach to demonstrate release of pituitary neuropeptides and the relation between secretoryprofiles of neuropeptides and those of pituitary hormones. It is another approach to all thesedifferent points than long term culture that needs enzymatic dispersion, and several days ofrecovery before any experiment can be performed on the cells.