Intraerythrocytic multiplication of Theileria parva in vitro: an ultrastructural study |
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| Authors: | P A Conrad D Denham C G Brown |
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| Affiliation: | 1. Department of Dermatology, Osaka City University Graduate School of Medicine, Osaka, Japan;2. Department of Dermatology, Keio University School of Medicine, Tokyo, Japan;3. Experimental Immunology, Osaka University Immunology Frontier Research Center, Osaka, Japan;1. School of Public Health, Shandong University, Jinan, Shandong Province, China;2. Department of Pathology, University of Texas Medical Branch, Galveston, TX 77555-0609, USA;1. State Key Laboratory of Veterinary Etiological Biology, Key Laboratory of Veterinary Parasitology of Gansu Province, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Gansu, PR China;2. Faculty of Veterinary Science, The University of Melbourne, Parkville, Victoria, Australia;1. University of Georgia, College of Veterinary Medicine, Department of Pathology, 501 D.W. Brooks Dr., Athens, GA, 30602, United States;2. University of Georgia, Center for Tropical and Emerging Global Diseases, Department of Infectious Diseases, 500 D.W. Brooks Dr., Athens, GA, 30602, United States;3. North Carolina State University, College of Veterinary Medicine, Department of Clinical Sciences, 1060 William Moore Drive, Raleigh, NC, 27607, United States;1. School of Veterinary Medicine, Faculty of Medical Sciences, University of the West Indies, Building 47, Eric Williams Medical Sciences Complex, Uriah Butler Highway, Champ Fleurs, Trinidad and Tobago;2. Department of Pathology, The University of Georgia, College of Veterinary Medicine, 501 D.W. Brooks Dr., Athens, GA 30602, United States |
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| Abstract: | Intraerythrocytic multiplication of Theileria parva in vitro: an ultrastructural study. International Journal for Parasitology16: 223–229. Piroplasms of a Theileria parva isolate from Muguga, Kenya were maintained in short-term stationary erythrocyte cultures and the mode of multiplication studied by light and electron microscopy. Division of single piroplasms into four intraerythrocytic merozoites was observed within the first 24 h in vitro. After 8 days of cultivation, 10–20% of the parasitised erythrocytes contained quadruplet merozoite forms resulting from multiplication in vitro. Electronmicrographs showed that intraerythrocytic multiplication occurred by a schizogonous process that proceeded from the initial formation of rhoptries and electron-dense cytoplasmic plaques beneath the parasite's plasmalemmal membrane, to nuclear division and the final separation of a maximum of four differentiated merozoites. The merozoites formed by intra-erythrocytic schizogony in vitro had ultrastructural features identical to merozoites produced by intra-lymphocytic schizonts of T. parva. |
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