| 桃脂氧合酶基因的原核表达分析 |
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| 引用本文： | 田英,刘廷旭,赵彩平,韩明玉,智军海.桃脂氧合酶基因的原核表达分析[J].西北植物学报,2011,31(3):499-503. |
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| 作者姓名： | 田英 刘廷旭 赵彩平 韩明玉 智军海 |
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| 作者单位： | 西北农林科技大学,园艺学院,陕西杨陵,712100 |
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| 基金项目： | 陕西省"13115"科技创新工程重大科技专项，陕西省科技攻关 |
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| 摘 要： | 利用RT-PCR技术从桃(Prunus persica L.)基因组中克隆到脂氧合酶(lipoxygenase,LOX)基因3(PpLOX-3)的ORF全长.ORF全长与原核表达载体pET-32a(+)相连接,构建重组原核表达载体pET-LOX-3,并转化大肠杆菌BL21(DE3)感受态细胞,在IPTG诱导下,通过SDS-PAGE电泳,得到一条约125 kD的融合蛋白条带,除去pET-32a(+)自身诱导的约20 kD大小的蛋白后,结果与PpLOX-3编码的约105 kD蛋白的大小一致.
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| 关 键 词： | 桃 脂氧合酶 原核表达 |
Expression of Peach PpLOX-3 in Prokaryotic Cells |
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| TIAN Ying,LIU Ting-xu,ZHAO Cai-ping,HAN Ming-yu,ZHI Jun-hai.Expression of Peach PpLOX-3 in Prokaryotic Cells[J].Acta Botanica Boreali-Occidentalia Sinica,2011,31(3):499-503. |
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| Authors: | TIAN Ying LIU Ting-xu ZHAO Cai-ping HAN Ming-yu ZHI Jun-hai |
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| Institution: | TIAN Ying,LIU Ting-xu,ZHAO Cai-ping,HAN Ming-yu,ZHI Jun-hai(College of Horticulture,Northwest A&F University,Yangling,Shaanxi 712100,China) |
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| Abstract: | PpLOX-3 gene was cloned by RT-PCR from Prunus persica genome.The PpLOX-3 was subcloned into the multiple clone sites of plasmid pET-32a(+) to get the prokaryotic vector of pET-LOX-3,which was transformed into E.coli BL21(DE3).The fusion protein PpLOX-3 was expressed under IPTG concentrations,according to SDS-PAGE analysis,PpLOX-3 fusion protein was expressed with molecular weight of 125 kD,remove the pET-32a(+)'s own induction produced 20 kD protein,the result is consistent with the 105 kD protein which enc... |
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| Keywords: | Prunus persica L lipoxygenase prokaryotic expression |
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