Growth effects and metabolism of arginine in stationary and rapidly-dividing sugarcane cell suspensions |
| |
| Authors: | Edward P Glenn |
| |
| Institution: | E. P. Glenn, Dept. of Botanical Sciences, University of Hawaii at Manoa, Honolulu, HI 96S2Z U.S.A. |
| |
| Abstract: | The effect of 300 μ M arginine on growth of sugarcane cell suspensions was studied. Cells transferred to defined media in the stationary growth stage showed a greater requirement for exogenous arginine than cells similarly transferred in the rapidly dividing stage. Cell arginine levels, rates of arginine synthesis, and enzymes of arginine synthesis all decreased in cells entering the stationary stage. It is concluded that stationary stage cells are deficient in their ability to synthesize arginine and are therefore dependent upon an exogenous supply to resume growth in fresh media. |
| |
| Keywords: | Arginine sugarcane cell cultures argininosuccinate synthetase arginmosuccinate lyase ornitbine transcarbamoylase differentiation |
|
|
