Effect of media composition on the induction of chorionic gonadotropin by sodium butyrate in HeLa cells |
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Authors: | G Stanley Cox Daniel S McClure |
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Institution: | (1) Department of Biochemistry and Biophysics and Program in Molecular, Cellular, and Developmental Biology, Iowa State University, 50011 Ames, Iowa |
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Abstract: | Summary Production of the glycoprotein hormone α-subunit by HeLa cells and its induction by sodium butyrate are dependent on the choice
of culture medium. Under identical growth conditions it was found that subunit synthesis in the presence of butyrate was highest
in RPMI 1640, lowest in Medium 199 (M199), and intermediate in minimum essential medium (MEM) and Waymouth's MB 752/1. Cell
growth was similar in all media examined and was retarded in the presence of butyrate. Alkaline phosphatase activity was also
lower in M199 than in RPMI 1640, although, in general, the magnitude of this difference was less than that for the hormone
subunit. Incorporation of 1-14C]butyrate by HeLa cells was simimar in both M199 and RPMI 1640, indicating that uptake and metabolism of the fatty acid were
not significantly different under these conditions. In the presence of 3 mM butyrate, mixtures of RPMI 1640 and M199 gave intermediate levels of α-subunit and alkaline phosphatase compared to each
medium alone. Intracellular levels of α-subunit as well as that of the culture medium were reduced in M199 compared to RPMI
1640 indicating that synthesis rather than secretion was altered.
This work was supported by Grant CA 21534 from the National Institutes of Health, Bethesda, MD. |
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Keywords: | chorionic gonadotropin HeLa cells sodium butyrate induction media composition |
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