| 金黄色葡萄球菌肠毒素B基因的克隆和在大肠杆菌中高效表达 |
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| 引用本文： | 杨立泉 吴文芳 时成波 吕安国 冯家勋 柏学亮. 金黄色葡萄球菌肠毒素B基因的克隆和在大肠杆菌中高效表达[J]. 生物工程学报, 2002, 18(5): 597-600 |
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| 作者姓名： | 杨立泉 吴文芳 时成波 吕安国 冯家勋 柏学亮 |
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| 作者单位： | 1. 中国科学院沈阳应用生态研究所,沈阳,110016
2. 广西大学分子遗传所 |
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| 基金项目： | 沈阳市科委项目 (No .2 0 0 12 2 1 0 3)，沈阳应用生态研究所与屹昌科技集团股份有限公司合作项目~~ |
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| 摘 要： | 利用PCR方法从金黄色葡萄球菌TSTw基因组DNA中扩增出约700bp的DNA片段，将之克隆到pGEM7Zf(+)载体上并转化大肠杆菌 DH5α菌株。重组质粒的测序结果表明克隆到了seb基因，它含有717bp（不包括N端81bp的信号肽编码区），其核苷酸序列与文献报道完全一致。将其连接于表达载体7ZTS上，转化到大肠杆菌JM109（DE3）内。表达的SEB占总蛋白33.5%。
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| 关 键 词： | SEB， 基因克隆和表达， 超抗原 |
| 文章编号： | 1000-3061(2002)05-0597-04 |
| 修稿时间： | 2002-04-12 |
Cloning of Staphylococcal Enterotoxin B Gene and Its Highly Expression in Escherichia coli |
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| YANG Li-Quan WU Wen-Fang+ SHI Cheng-Bo LU An-Guo FENG Jia-Xun BAI Xue-Liang. Cloning of Staphylococcal Enterotoxin B Gene and Its Highly Expression in Escherichia coli[J]. Chinese journal of biotechnology, 2002, 18(5): 597-600 |
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| Authors: | YANG Li-Quan WU Wen-Fang+ SHI Cheng-Bo LU An-Guo FENG Jia-Xun BAI Xue-Liang |
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| Affiliation: | Shenyang Institute of Applied Ecology, Chinese Academy of Science, Shenyang 110015, China. |
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| Abstract: | An about 700 bp DNA fragment was amplified from genome DNA of S. aureus TSTw by PCR. This fragment was cloned into pGEM-7Zf(+) and the recombinant plasmid was transformed into E. coli DH5 alpha. The sequencing result of the recombinant plasmid demonstrated that it contains seb gene with 717 bp (without signal encoding region of 81 bp) which has the same nucleotide sequence as described in literature. The seb gene was cloned into expression vector 7ZTS and was transformed into E. coli JM109 (DE3). The expression level of SEB was as high as 33.3% of the cell total proteins. |
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| Keywords: | SEB gene cloning and expression superantigen |
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