Isolation and characterization of xylans from seed pericarp of Argania spinosa fruit |
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Authors: | Habibi Youssef Vignon Michel R |
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Affiliation: | Centre de Recherches sur les Macromolécules Végétales, C.N.R.S., affiliated with the Joseph Fourier University of Grenoble, BP 53, 38041 Grenoble cedex 9, France. |
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Abstract: | Hemicellulose-type polysaccharides were isolated from the pericarp of seeds of Argania spinosa (L.) Skeels fruit by sequential alkaline extractions and fractionated by precipitation. Water soluble and water insoluble fractions were obtained, purified and characterized by sugar analysis and 1H and 13C NMR spectroscopy. The water soluble fractions were assumed to be (4-O-methyl-D-glucurono)-D-xylans, with 4-O-methyl-D-glucopyranosyluronic acid groups linked to C-2 of a (1-->4)-beta-D-xylan. The 1H NMR spectrum showed that the water soluble xylans have, on average, one non-reducing terminal residue of 4-O-methyl-D-glucuronic acid for every seven xylose units. The water insoluble fractions consisted of a neutral xylan with linear (1-->4)-beta-D-xylopyranosyl units. |
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Keywords: | Argania spinosa Seed (1→4)-β- smallcaps" >d-Xylan 1H and 13C NMR spectroscopy |
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