莴苣花粉发育过程中ATP酶的分布

对莴苣花粉发育过程中ATPase的分布特征做了研究。四分体早期的小孢子细胞质中开始出现ATPase反应颗粒。之后,小孢子在发育过程中,花粉内壁聚集大量体积较大的ATPase反应颗粒,并一直保持到花粉即将成熟。在小孢子发育晚期,在花粉萌发孔处和小孢子大液泡中也特异性地聚集了较多ATPase颗粒。二胞花粉刚形成的生殖细胞表面呈现大量的ATPase反应颗粒,当生殖细胞脱离花粉内壁移入营养细胞,ATPase反应颗粒基本消失。生殖细胞分化过程中生殖细胞的ATPase反应颗粒逐渐低于营养细胞中的。在成熟花粉中,精细胞中的ATPase反应颗粒比营养细胞中的少,且主要集中在细胞核中。结果显示花粉发育过程中ATPase的特异分布与花粉发育的一些生物学事件密切相关。

Localization of ATPase during Pollen Development in Lattuce sativa L.

The localization of ATPase in developing pollen grains of Lattuce sativa L. was studied using lead precipitation. ATPase precipitates (ppts) were present in the microspore cell during the early tetrad stage. Ppts then occurred largely in the inner pollen wall until shortly before pollens formed. At the late microspore developmental stage, abundant ppts were found specifically at the germ pore and in the large vacuole of microspore. During two-celled pollen grain stage, they were accumulated on the wall of the generative cell. After the generative cell came into the vegetative cell, ppts largely disappeared. The number of ppts was less in the generative cell than in the vegetation cell during the differentiation of the generative cell. And it was less in sperm cells than in the vegetation cell after pollen formed. It is suggested that the distribution of ATPase is closely related to events of pollen development.