| 基于组合胶体金纳米颗粒的免疫层析试纸条快速可视化检测海产品中副溶血性弧菌 |
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| 引用本文： | 吴美娇,吴有雪,刘程,田亚晨,方水琴,刘箐.基于组合胶体金纳米颗粒的免疫层析试纸条快速可视化检测海产品中副溶血性弧菌[J].工业微生物,2021,51(2):1-9. |
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| 作者姓名： | 吴美娇 吴有雪 刘程 田亚晨 方水琴 刘箐 |
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| 作者单位： | 上海理工大学医疗器械与食品学院,上海200093;上海理工大学医疗器械与食品学院,上海200093;青岛海洋科学与技术试点国家实验室,海洋渔业科学与食物产出过程功能实验室,青岛266071 |
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| 基金项目： | 国家重点研发计划新型生物识别材料库的构建及其制备关键技术研究(2018YFC1602900);国家自然科学基金(31871897);上海市科技创新行动计划生鲜肉制品食源性致病菌的“可视化”智能互联快速检测产品研发(19391902000)。 |
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| 摘 要： | 本研究以野生型副溶血性弧菌(Vibrio parahaemolyticus)为免疫原制备单克隆抗体,并以组合胶体金纳米粒子(CP-AuNPs)为抗体标记探针,开发了一种基于双抗体夹心原理的可视化快速检测副溶血性弧菌的免疫层析试纸条方法.该方法被应用于梅子鱼、鲜虾、白蛤等海产品中副溶血性弧菌的检测,具有特异性强、检测时间...
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| 关 键 词： | 副溶血性弧菌 单克隆抗体 组合胶体金纳米粒子 免疫层析试纸条 海产品 |
Rapid visual detection of Vibrio parahaemolyticus in seafood by immunochromatographic strip based on composited colloidal gold nanoparticles |
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| WU Meijiao,WU Youxue,LIU Cheng,TIAN Yachen,FANG Shuiqin,LIU Qing.Rapid visual detection of Vibrio parahaemolyticus in seafood by immunochromatographic strip based on composited colloidal gold nanoparticles[J].Industrial Microbiology,2021,51(2):1-9. |
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| Authors: | WU Meijiao WU Youxue LIU Cheng TIAN Yachen FANG Shuiqin LIU Qing |
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| Institution: | (School of Medical Instrument and Food Engnieering, University of Shanghai for Science and Technology, Shanghai 200093;Laboratory for Marine Fisheries Science and Food Production Processes, Qingdao National Laboratory for Marine Science and Technology, Qingdao, Shandong 266071) |
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| Abstract: | Using wild type Vibrio parahaemolyticus as immunogen to prepare monoclonal antibody and composited colloidal gold nanoparticles(CP-AuNPs)as antibody-labeled probes,an immunochromatography test strip method for visual and rapid detection of Vibrio parahaemolyticus based on double antibody sandwich principle was developed. This method was applied to the detection of Vibrio parahaemolyticus in seafood,including plum fish,fresh shrimps,white clams etc,with the advantages of strong specificity,short detection time and high sensitivity,and its detection limit could reach 4. 77 ×103 CFU/mL. As a result,this method could be used as an effective and rapid diagnostic tool for detecting Vibrio parahaemolyticus in seafood. |
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| Keywords: | Vibrio parahaemolyticus monoclonal antibody composited colloidal gold nanoparticles(CP-AuNP) immunochromatographic assay seafood products |
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