Targeted gene disruption of the avermectin B O-methyltransferase gene in Streptomyces avermitilis |
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| Authors: | Young-Soo Hong Jeong Hyung Lee Hang Sub Kim Kyu-Won Kim and Jung Joon Lee |
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| Institution: | (1) Korea Research Institute of Bioscience and Biotechnology, P.O. Box, 115, Yusong, Taejon, 305-600, Korea;(2) Department of Molecular Biology, Pusan National University, Pusan, 609-735, Korea |
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| Abstract: | The biological activity of avermectin B components is superior to that of avermectin A components, which are derived from avermectin B by avermectin B 5-O-methyltransferase. Gene disruption, targeting avermectin B 5-O-methyltransferase gene in Streptomyces avermitilis, was carried out to obtain a strain of avermectin B producer. Phenotype analysis of the mutant with the disrupted O-methyltransferase gene showed that only avermectin B components were produced with a significant increase in production |
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| Keywords: | avermectin avermectin O-methyltransferase gene disruption |
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