Microtubules in pollen tube subprotoplasts: organization during protoplast formation and protoplast outgrowth |
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Authors: | T L M Rutten J Derksen |
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Institution: | (1) Department of Experimental Botany, University of Nijmegen, Toernooiveld, NL-6525 ED Nijmegen, The Netherlands |
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Abstract: | Summary Microtubules inNicotiana tabacum pollen tube subprotoplasts reassembled in wave-like to concentric cortical arrays. Crosslinks between microtubules were either 15 or 80 nm in length. Cortical actin filaments showed different distributions; no colocalization like that in pollen tubes was observed. Degradation of actin filaments by cytochalasin D had no influence on microtubule organization. Degradation of microtubules and/or actin filaments did not affect outgrowth of the subprotoplasts. Organization of the microtubules occurred independent of the presence of the generative cell and/or the vegetative nucleus. No relation of actin filament and microtubule organization with organelle distribution could be detected.Abbreviations AFs
actin filaments
- DAPI
4 ,6-diamidino-2-phenylindole
- EGTA
ethylene glycol bis (2-amino ethylether) N,N,N ,N -tetraacetic acid
- FITC
fluorescein isothiocyanate
- MTs
microtubules
- SPPs
subprotoplasts
- TRITC
tetramethyl rhodamine B isothiocyanate |
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Keywords: | Actin filaments Microtubules Nicotiana tabacum Pollen tube Protoplast |
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