L-MBP is expressed in epithelial cells of mouse small intestine |
| |
Authors: | Uemura Kazuhide Saka Masako Nakagawa Tomoaki Kawasaki Nobuko Thiel Steffen Jensenius Jens Christian Kawasaki Toshisuke |
| |
Affiliation: | Department of Biological Chemistry, Core Research for Science and Technology Project, Japan Science and Technology Corp., Graduate School of Pharmaceutical Sciences, Kyoto University, Japan. |
| |
Abstract: | The mannan-binding proteins (L-MBP and S-MBP, also denoted MBL-C and MBL-A), mainly produced in liver and existing in liver and serum, play important roles in the innate immunity against a variety of pathogens. Total RNA from mouse tissues were screened for MBP mRNA by RT-PCR. In addition to liver, S-MBP mRNA was detected in lung, kidney, and testis, and L-MBP mRNA was detected in kidney, thymus, and small intestine. Quantitative RT-PCR revealed that the small intestine is a predominant site of extrahepatic expression of L-MBP. Western blotting with polyclonal Abs against rat L-MBP demonstrated this protein in Triton X-100 extracts of the small intestine obtained from mice that had undergone systemic perfusion. Immunohistochemical staining with an mAb against mouse L-MBP and in situ hybridization revealed that L-MBP is selectively expressed in some villous epithelial cells of the small intestine. These findings suggest that L-MBP plays a role in mucosal innate immunity. |
| |
Keywords: | |
本文献已被 PubMed 等数据库收录! |
|