Use of central composite experimental design for the development ofBradyrhizobium japonicum liquid inoculants |
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| Authors: | Sophie Cliquet Christine Durier Gérard Catroux |
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| Affiliation: | (1) Laboratoire de Microbiologie des Sols, BV 1540, INRA, 21034 DIJON Cedex, France;(2) Station de Biométrie, INRA, Route de St Cyr, 78026 Versailles Cedex, France |
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| Abstract: | Central composite experimental designs realized with flask cultures and completed by fermentor studies were used to developB. japonicum liquid inoculants. Two different media are described, using glycerol or glucose, in the presence of only 1 g/l yeast extract. They allow the production of cultures containing more than 1010 viable cell/ml, able to nodulate soybean efficiently. |
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