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| 植物高度木质化叶片表皮细胞制备方法 | |
| 引用本文： | 吴清韩,赵庆芳,朱慧,马瑞君. 植物高度木质化叶片表皮细胞制备方法[J]. 植物学通报, 2012, 47(4): 422-426 |
| 作者姓名： | 吴清韩 赵庆芳 朱慧 马瑞君 |
| 作者单位： | 1. 韩山师范学院资源植物研究所,潮州521041;西北师范大学生命科学学院,兰州730070 2. 西北师范大学生命科学学院,兰州,730070 3. 韩山师范学院资源植物研究所,潮州,521041 |
| 基金项目： | 基金项目：广东省科技计划（No.20118020304012） |
| 摘 要： | 以凤凰茶(Camellia sinensis)叶片为例,介绍一种获取高度木质化植物叶片表皮细胞的简易方法——叶片燃烧法.该方法根据高度木质化的叶片在燃烧、过水之后,其上下表皮自行分开、细胞透明化,从而获得材料上下表皮细胞的轮廓.该方法具有简单迅速、制片效率高、上下表皮易区分及可靠稳定等优点,非常适用于木质化叶片表皮特征的研究,也可为植物学及生态学的相关研究方法提供参考. |
| 关 键 词： | 燃烧 表皮细胞 凤凰茶 叶片 木质化 |
A Method to Improve Sample Preparation for Observing Epidermis of Highly Lignified Leaf |
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| Qinghan Wu , Qingfang Zhao , Hui Zhu , Ruijun Ma. A Method to Improve Sample Preparation for Observing Epidermis of Highly Lignified Leaf[J]. Chinese Bulletin of Botany, 2012, 47(4): 422-426 | |
| Authors: | Qinghan Wu Qingfang Zhao Hui Zhu Ruijun Ma |
| Affiliation: | 1Institute of Plant Resources, Hanshan Normal University, Chaozhou 521041, China; 2College of Life Sciences, Northwest Normal University, Lanzhou 730070, China |
| Abstract: | We have developed an efficient leaf-burning method to observe epidermal cells of tea which are highly lignified in cell walls. With this method, the clear shape of the upper and lower epidermis cells can be observed after several steps of burning and rinsing. After treatment, the upper and lower epidermis cells can automatically separate and hyalinize. This method is easy to use, saves time, distinguishes the upper from the lower side of plant epidermis and has high reliability and stability. The epidermal characteristics of the lignified leaves can be accurately and quickly determined for botany and ecology research. |
| Keywords: | burning epidermal cell fenghuang tea leaves lignified |
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