Expression of nestin,desmin and vimentin in intact and regenerating muscle spindles of rat hind limb skeletal muscles |
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Authors: | Dana Čížková Tomáš Soukup Jaroslav Mokrý |
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Institution: | (1) Department of Histology and Embryology, Faculty of Medicine in Hradec Kralove, Charles University in Prague, Šimkova 870, Hradec Králové, 500 38, Czech Republic;(2) Institute of Physiology, Academy of Sciences of the Czech Republic, Vídeňská 1083, 142 20 Prague 4, Czech Republic |
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Abstract: | We describe the expression and distribution patterns of nestin, desmin and vimentin in intact and regenerating muscle spindles
of the rat hind limb skeletal muscles. Regeneration was induced by intramuscular isotransplantation of extensor digitorum
longus (EDL) or soleus muscles from 15-day-old rats into the EDL muscle of adult female inbred Lewis rats. The host muscles
with grafts were excised after 7-, 16-, 21- and 29-day survival and immunohistochemically stained. Nestin expression in intact
spindles in host muscles was restricted to Schwann cells of sensory and motor nerves. In transplanted muscles, however, nestin
expression was also found in regenerating “spindle fibers”, 7 and 16 days after grafting. From the 21st day onwards, the regenerated
spindle fibers were devoid of nestin immunoreactivity. Desmin was detected in spindle fibers at all developmental stages in
regenerating as well as in intact spindles. Vimentin was expressed in cells of the outer and inner capsules of all muscle
spindles and in newly formed myoblasts and myotubes of regenerating spindles 7 days after grafting. Our results show that
the expression pattern of these intermediate filaments in regenerating spindle fibers corresponds to that found in regenerating
extrafusal fibers, which supports our earlier suggestion that they resemble small-diameter extrafusal fibers. |
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Keywords: | Intermediate filaments Muscle spindle regeneration Skeletal muscle transplantation Rat muscle spindles Rat hind limb |
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