Changes in hydroxy and carboxylic acid compositions in the supernatant fluids of mosquito cell cultures infected with dengue viruses

Hydroxy and carboxylic acids in the supernatant fluids of mosquito cell cultures infected with four serotypes of dengue viruses (DEN) were analyzed by frequency-pulsed electron capture gasliquid chromatography. The hydroxy acid profiles of all virus-infected cell cultures differed qualitatively and quantitatively from the profile of normal cell culture. Furthermore, the profiles of hydroxy acids in the DEN 1- and DEN 4-infected cultures were type specific. Although quantitative differences of a few peaks could be found between the hydroxy acid profiles of DEN 2- and DEN 3-infected cultures, in the absence of clear qualitative differences the two profiles were considered to be essentially indistinguishable. The carboxylic acid profiles of virus-infected cultures differed from the profile of a normal cell culture, but none of the four serotypes of DEN viruses induced type-specific profiles. Thus, these findings contrasted to previous results with rhesus monkey kidney cell cultures (LLC-MK₂), in which serotype-specific sets of hydroxy acids and a DEN 1-specific set of carboxylic acids were released in the supernatant fluids by the infection with dengue viruses.