Comparison of different approaches for the incorporation of non-radioactive labels into polymerase chain reaction products |
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| Authors: | Martine Gauthier Burton W Blais |
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| Institution: | (1) Ottawa Laboratory (Carling), Canadian Food Inspection Agency, Ottawa, Canada, K1A 0C6 |
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| Abstract: | Different methods for labelling polymerase chain reaction (PCR) products with non-radioactive labels for their detection by hybridization with immobilized DNA probes were compared. The use of digoxigenin (DIG) as a label provided greater sensitivity than biotin in a PCR system targeting the invA gene from Salmonella typhimurium. Incorporation of digoxigenin into amplicons in the form of 5 -DIG-labelled oligonucleotide primers resulted in better assay signals and was more economical than DIG-labelled dUTP. |
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| Keywords: | biotin digoxigenin hybridization polymerase chain reaction Salmonella typhimurium |
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