Cloning of heat-shock locus 93D from Drosophila melanogaster. |
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Authors: | U Walldorf, S Richter, R P Ryseck, H Steller, J E Edstr m, E K Bautz, B Hovemann |
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Affiliation: | U Walldorf, S Richter, R P Ryseck, H Steller, J E Edström, E K Bautz, and B Hovemann |
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Abstract: | Using the microcloning approach a number of recombinant lambda phages carrying DNA from the 93D region have been isolated. Screening genomic libraries, cloned in phage lambda or cosmid vectors, with this isolated DNA yielded a series of overlapping DNA fragments from the region 93D6-7 as shown by in situ hybridization to polytene chromosomes. In vitro 32P-labelled nuclear RNA prepared from heat-shocked third instar larvae hybridized specifically to one fragment within 85 kb of cloned DNA. The region which is specifically transcribed after heat shock could be defined to a cluster of internally-repetitive DNA and its neighbouring proximal sequences. Over a sequence of 10-12 kb in length the DNA is cut into repeat units of approximately 280 nucleotides by the restriction endonuclease TaqI. The TaqI repeat sequences are unique in the Drosophila genome. |
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