Molecular cloning and expression of Chimonanthus praecox farnesyl pyrophosphate synthase gene and its possible involvement in the biosynthesis of floral volatile sesquiterpenoids |
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| Authors: | Lin Xiang Kaige Zhao Longqing Chen |
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| Institution: | 1. Key Laboratory of Horticultural Plant Biology (Ministry of Education), College of Horticulture and Forestry Sciences, Huazhong Agricultural University, Wuhan 430070, PR China;2. Institute of Horticulture, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, PR China;1. Department of Ornamental Horticulture, School of Landscape Architecture, Zhejiang Agriculture and Forestry University, 311300 Lin’an Hangzhou, China;2. Nurturing Station for State Key Laboratory of Subtropical Silviculture, 311300 Lin’an Hangzhou, China;1. College of Materials and Environmental Engineering, Hangzhou Dianzi University, Hangzhou 310018, PR China;2. Artistic Design & Creation School, Zhejiang University City College, Hangzhou 310015, PR China;3. Hangzhou Botanical Garden, Hangzhou, Zhejiang 310013, PR China;4. Institute of Botany, Jiangsu Province and Chinese Academy of Sciences, Nanjing Botanical Garden, Mem. Sun Yat-Sen, Nanjing 210014, PR China;5. Key Laboratory of Marine New Materials and Related Technology, Zhejiang Key Laboratory of Marine Materials and Protection Technology, Ningbo Institute of Material Technology and Engineering, Chinese Academy of Sciences, Ningbo 315201, PR China;6. Department of Chemistry and Biotechnology, Faculty of Science, Engineering and Technology, Swinburne University of Technology, Hawthorn, VIC 3122, Australia;1. Department of Applied Chemistry, Zhejiang Gongshang University, Hangzhou 310018, PR China;2. College of Pharmaceutical Sciences, Zhejiang University of Technology, Hangzhou 310014, PR China;3. Analysis & Testing Center, Soochow University, Suzhou 215123, Jiangsu, PR China;4. Ocean College, Zhejiang University, Hangzhou 310058, PR China;1. Plant Molecular Biology Unit, Division of Biochemical Sciences, National Chemical Laboratory, Pune 411 008, India;2. Department of Molecular Ecology, Max Planck Institute for Chemical Ecology, Beutenberg Campus, D-07745 Jena, Germany;3. Department of Biochemistry, Max Planck Institute for Chemical Ecology, Beutenberg Campus, D-07745 Jena, Germany;4. Dr. Balasaheb Sawant Konkan Agriculture University, Dapoli 415712, India;1. Guiyang College of Traditional Chinese Medicine, Guiyang 550002, China;2. State Key Laboratory of Functions and Applications of Medicinal Plants, Guizhou Medical University, Guiyang 550014, China;3. The Key Laboratory of Chemistry for Natural Products of Guizhou Province and Chinese Academy of Sciences, Guiyang 550014, China;1. Department of Plant & Microbial Biology, North Carolina State University, Raleigh, NC, 27695, United States;2. State Key Laboratory of Tree Genetics and Breeding, Research Institute of Forestry, Chinese Academy of Forestry, Key Laboratory of Tree Breeding and Cultivation of the State Forestry Administration, Beijing, 100091, China |
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| Abstract: | Farnesyl pyrophosphate (FPP) synthase catalyzes the biosynthesis of FPP, which is the precursors of sesquiterpenoids such as floral scent volatiles, from isopentenyl pyrophosphate (IPP) and dimethylallyl pyrophosphate (DMAPP). cDNA encoding wintersweet (Chimonanthus praecox L.) FPP synthase was isolated by the RT-PCR and RACE methods. The deduced amino acid sequence showed a high identity to plant FPP synthases. Expression of the gene in Escherichia coli yielded FPPS activity that catalyzed the synthesis of FPP as a main product. Tissue-specific and developmental analyses of the mRNA levels of CpFPPS and volatile sesquiterpenoids levels in C. praecox flowers revealed that the FPPS may play a regulatory role in floral volatile sesquiterpenoids of wintersweet. |
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