Life-cycle chronic gamma exposure of Arabidopsis thaliana induces growth effects but no discernable effects on oxidative stress pathways |
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| Authors: | Hildegarde Vandenhove Nathalie Vanhoudt Ann Cuypers May van Hees Jean Wannijn Nele Horemans |
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| Affiliation: | 1. Belgian Nuclear Research Centre (SCK·CEN), Biosphere Impact Studies, Boeretang 200, 2400 Mol, Belgium;2. Hasselt University, Centre for Environmental Sciences, Agoralaan Building D, B-3590 Diepenbeek, Belgium;1. Departamento de Bioquímica, Biología Celular y Molecular de Plantas, Estación Experimental del Zaidin, CSIC, C/Prof. Albareda 1, Granada E-18008, Spain;2. Department of Biology, Faculty of Science, Ehime University, Matsuyama 790-8577, Japan;1. SCK·CEN, Biosphere Impact Studies, Boeretang 200, 2400 Mol, Belgium;2. Centre for Environmental Research, Universiteitslaan 1, 3590 Diepenbeek, Belgium;1. Department of Biology, Institute of Nuclear Power Engineering NRNU MEPhI, Studgorodok,1, Obninsk, Kaluga Region 249040, Russian Federation;2. National Research Nuclear University “MEPhI”, Kashirskoe Highway, 31, Moscow 115409, Russian Federation;3. Department of Genetics, University of Leicester, University Road, Leicester LE1 7RH, United Kingdom;4. Vavilov Institute of General Genetics, Russian Academy of Sciences, Gubkina Str. 3, 11933 Moscow, Russian Federation;1. Institut de Radioprotection et de Sûreté Nucléaire (IRSN), PRP-ENV/SERIS/LECO, Cadarache, St Paul-lez-Durance 13115, France;2. UMR 5805 EPOC – OASU, Station marine d’Arcachon, Université Bordeaux 1, Arcachon 33120, France;1. SCK?CEN, Boeretang 200, 2400 Mol, Belgium;2. Department of Biology, University of Antwerp, Groenenborgerlaan 171, 2020 Antwerpen, Belgium;3. Veterinary Sciences, University of Antwerp, Groenenborgerlaan 171, 2020 Antwerpen, Belgium;4. Centre for Environmental Research, University of Hasselt, Universiteitslaan 1, 3590 Diepenbeek, Belgium |
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| Abstract: | Arabidopsis thaliana was exposed to low-dose chronic gamma irradiation during a full life cycle (seed to seed) and several biological responses were investigated. Applied dose rates were 2336, 367 and 81 μGy h?1. Following 24 days (inflorescence emergence), 34 days (～50% of flowers open) and 54 days (silice ripening) exposure, plants were harvested and monitored for biometric parameters, capacities of enzymes involved in the antioxidative defence mechanisms (SOD, APOD, GLUR, GPOD, SPOD, CAT, ME), glutathione and ascorbate pool, lipid peroxidation products, altered gene expression of selected genes encoding for antioxidative enzymes or reactive oxygen species production, and DNA integrity. Root fresh weight was significantly reduced after gamma exposure compared to the control at all stages monitored but no significant differences in root weight for the different dose rates applied was observed. Leaf and stem fresh weight were significantly reduced at the highest irradiation level after 54 days exposure only. Also total plant fresh was significantly lower at silice riping and this for the highest and medium dose rate applied. The dose rate estimated to result in a 10% reduction in growth (EDR-10) ranged between 60 and 80 μGy h?1. Germination of seeds from the gamma irradiated plants was not hampered. For several of the antioxidative defence enzymes studied, the enzyme capacity was generally stimulated towards flowering but generally no significant effect of dose rate on enzyme capacity was observed. Gene analysis revealed a significant transient and dose dependent change in expression of RBOHC indicating active reactive oxygen production induced by gamma irradiation. No effect of irradiation was observed on concentration or reduction state of the non-enzymatic antioxidants, ascorbate and glutathione. The level of lipid peroxidation products remained constant throughout the observation period and was not affected by dose rate. The comet assay did not reveal any effect of gamma dose rate on DNA integrity. |
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