Prolonged stability of the plantibody HB-01 directed against the hepatitis B surface antigen in cryo-preserved tobacco leaves |
| |
Authors: | Williams Ferro Déborah Geada Yarysel Guevara Sigifredo Padilla Adrián Conde Tatiana Alvarez Yenisley Medina Tatiana González Mayra Wood Rodolfo Valdés |
| |
Affiliation: | 1. Monoclonal Antibody Production Department, Center for Genetic Engineering and Biotechnology, Ave 31/158 and 190, Havana 10600, P.O. Box 6162, Cuba;2. Tobacco Research Institute, Tumbadero Road 8½ km, San Antonio de los Baños, Havana 3500, Cuba;3. Process Control Department, Center for Genetic Engineering and Biotechnology, Ave 31/158 and 190, Havana 10600, P.O. Box 6162, Cuba |
| |
Abstract: | Since 1983, several recombinant antibodies have been expressed in important agronomic plant species. However, to date no evaluation has been published about prolonged antibody stability within plant tissues under cryo-preservation conditions. This current report presents an approach to the KDEL-plantibody HB-01 (PHB-01) stability in frozen tobacco leaves by presenting scientific evidence about the stability of a plantibody to a prolonged low temperature exposure in this biological source. Results clearly show that the PHB-01 amount is maintained during the storage of tobacco leaves at ?20 °C for 90 days. The PHB-01 recovery was not affected by any irreversible physical and/or chemical change produced in tobacco leaves after this cryo-preservation time. The amount of total soluble proteins in the clarified extract decreased in proportion with the storage time and the PHB-01 molecules isolated from frozen leaf extracts were highly pure, >95%, according to an SDS-PAGE assessment under reducing conditions. Low temperature exposure of tobacco leaves did not reveal visible changes in frozen leaves, which is essential for the further extractability of proteins. The PHB-01 is stable in tobacco leaves at ?20 °C during 90 days, which offers the possibility to overcome problems associated with detrimental climate conditions and optimize purification capabilities. |
| |
Keywords: | |
本文献已被 ScienceDirect 等数据库收录! |
|