High efficiency transport of quantum dots into plant roots with the aid of silwet L-77 |
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| Authors: | Yong Hu Jun Li Lu Ma Qionglin Peng Wei Feng Lu Zhang Shibin He Fei Yang Jing Huang Lijia Li |
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| Institution: | 1. Departamento de Biodiversidad y Biología Experimental e Instituto de Biodiversidad y Biología Experimental y Aplicada (IBBEA, CONICET-UBA), Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Intendente Güiraldes 2160, Ciudad Universitaria, Pabellón II, (C1428EGA) Buenos Aires, Argentina;2. CERZOS-CONICET, Camino La Carrindanga Km 7, (8000) Bahía Blanca, Argentina;1. Department of Crop Science, North Carolina State University, Raleigh, NC 27695-7620, USA;2. DuPont Pioneer, 7250 NW 62nd Avenue, Johnston, IA 50131, USA;1. Plant Breeding and Acclimatization Institute - National Research Institute, Department of Plant Biochemistry and Physiology, Radzików, 05-870 B?onie, Poland;2. Department of Plant Molecular Ecophysiology, Faculty of Biology, Institute of Plant Experimental Biology and Biotechnology, University of Warsaw, 02-096 Warsaw, Miecznikowa 1, Poland;1. Key Lab of Plant Nutrition and Fertilizers, Ministry of Agriculture/Institute of Agricultural Resources and Regional Planning, Chinese Academy of Agricultural Sciences, Beijing 100081, P.R. China;2. College of Land and Environment, Shenyang Agricultural University, Shenyang 110866, P.R. China;1. State Key Laboratory of Pollution Control and Resources Reuse, School of the Environment, Nanjing University, Nanjing, 210023, PR China;2. Key Laboratory of Soil Environment and Pollution Remediation, Institute of Soil Science, Chinese Academy of Sciences, Nanjing, 210008, PR China;3. Environmental and Resource Studies Program (ERS), Trent University, Peterborough, Ontario, Canada;4. Environmental and Life Sciences Program (EnLS), Trent University, Peterborough, Ontario, Canada |
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| Abstract: | Quantum dots (QDs) are a novel type of small, photostable and bright fluorophores that have been successfully applied to mammalian and human live cell imaging. In this study, highly dispersive water-soluble mercaptoacetic acid (MAA)-coated CdSe/ZnS QDs were synthesized, which were suitable for investigation as fluorescent probe labels. The treatment of maize seedling roots with QDs showed that the surfactant silwet L-77 aided the efficient transport of QDs into maize roots. Under a concentration ranging from 0.128 to 1.28 μM, QDs caused very low cytotoxicity on maize seed germination and root growth. The addition of mercuric chloride to the Hoagland solution resulted in a decrease of QD content in root tissues, and this decrease was reversed upon the addition of β-mercaptoethanol, which suggests that mercury-sensitive processes play a significant role in regulating QD flow in the maize root system. We speculate that the apoplastic pathway can contribute substantially to the total quantity of QDs reaching the stele. Therefore, based on this transport approach, MAA-coated QDs can be utilized for live imaging in plant systems to verify known physiological processes. |
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