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Antinociceptive and neurochemical effects of a single dose of IB-MECA in chronic pain rat models |
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| Authors: | Cioato Stefania Giotti Medeiros Liciane Fernandes Lopes Bettega Costa de Souza Andressa Medeiros Helouise Richardt Assumpção José Antônio Fagundes Caumo Wolnei Roesler Rafael Torres Iraci L S |
| Institution: | 1.Laboratório de Farmacologia da Dor e Neuromodula??o, Investiga??es Pré-clínicas, Centro de Pesquisa Experimental (CPE), Hospital de Clínicas de Porto Alegre (HCPA), Universidade Federal do Rio Grande do Sul (UFRGS), Rua Ramiro Barcelos, 2350, Porto Alegre, RS, 90035-007, Brazil ;2.Unidade de Experimenta??o Animal, Grupo de Pesquisa e Pós-Gradua??o, HCPA, Porto Alegre, RS, Brazil ;3.Programa de Pós-Gradua??o em Ciências Biológicas, Farmacologia e Terapêutica, Instituto de Ciências Básicas da Saúde (ICBS), UFRGS, Porto Alegre, RS, Brazil ;4.Programa de Pós-Gradua??o em Saúde e Desenvolvimento Humano, Universidade La Salle, Canoas, RS, Brazil ;5.Programa de Pós-Gradua??o em Ciências Biológicas: Fisiologia, ICBS, UFRGS, Porto Alegre, RS, Brazil ;6.Programa de Pós-Gradua??o em Medicina, Ciências Médicas, UFRGS, Porto Alegre, RS, Brazil ;7.Cancer and Neurobiology Laboratory, Experimental Research Center, HCPA, Porto Alegre, RS, Brazil ; |
| Abstract: | This study aimed to evaluate the effect of a single administration of IB-MECA, an A3 adenosine receptor agonist, upon the nociceptive response and central biomarkers of rats submitted to chronic pain models. A total of 136 adult male Wistar rats were divided into two protocols: (1) chronic inflammatory pain (CIP) using complete Freund’s adjuvant and (2) neuropathic pain (NP) by chronic constriction injury of the sciatic nerve. Thermal and mechanical hyperalgesia was measured using von Frey (VF), Randal-Selitto (RS), and hot plate (HP) tests. Rats were treated with a single dose of IB-MECA (0.5 μmol/kg i.p.), a vehicle (dimethyl sulfoxide—DMSO), or positive control (morphine, 5 mg/kg i.p.). Interleukin 1β (IL-1β), brain-derived neurotrophic factor (BDNF), and nerve growth factor (NGF) levels were measured in the brainstem and spinal cord using enzyme-linked immunosorbent assay (ELISA). The establishment of the chronic pain (CIP or NP) model was observed 14 days after induction by a decreased nociceptive threshold in all three tests (GEE, P? |
| Keywords: | Inflammatory pain Neuropathic pain Adenosine receptor Rats Biomarkers |
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