A 22-amino-acid peptide restores DNA-binding activity to dimerization-defective mutants of the estrogen receptor. |
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Authors: | J A Lees S E Fawell R White M G Parker |
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Affiliation: | Molecular Endocrinology Laboratory, Imperial Cancer Research Fund, London, United Kingdom. |
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Abstract: | We have identified residues within the estrogen receptor that are required for dimerization and high-affinity DNA binding. A 22-amino-acid peptide encompassing these residues was sufficient to restore DNA-binding activity to a mutant receptor lacking most of the hormone-binding domain. Point mutagenesis of the fusion protein confirmed that this sequence continued to mediate dimerization in a manner similar to that within the native receptor, although its position relative to the DNA-binding domain was appreciably altered. |
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