Kinetic analysis of bioconversion of cellulose in attrition bioreactor |
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Authors: | Jones E O Lee J M |
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Institution: | Department of Chemical Engineering, Washington State University, Pullman, Washington 99164. |
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Abstract: | The attrition bioreactor (ABR) combines wet ball milling and enzymatic hydrolysis in one process step. It was found that the ABR did not accelerate enzyme deacti-vation. Interfacial forces, not shear forces, caused the most deactivation. Elimination of the air-liquid interface by covering the reactor substantially increased enzyme stability. A simple exponential kinetic model was tested to predict the cellulose conversion in an ABR. Kinetic parameters were estimated from batch runs performed at various enzyme and substrate concentrations. |
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