Microcinematographic demonstration of synchronous and asynchronous myoepithelial contractions in mouse submandibular gland rudiments in organotypic culture |
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Authors: | Willie D Morgan Jeannette E Williams Cecil W Lee Clyde J Dawe |
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Institution: | (1) Laboratory of Pathology, National Cancer Institute, National Institutes of Health, 20205 Bethesda, Maryland |
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Abstract: | Summary Time-lapse phase-contrast cinematography revealed contractile activity within mouse submandibular salivary gland rudiments
in organotypic culture. Three types of contraction were distinguishable. In type I (voiding contractions), all portions of
the gland contracted synchronously, and the active state ranged from 30 min to 2 hr. In type II (priming contractions), all
portions of the gland contracted synchronously, but the active state was shorter, ranging from 4 to 10 min. In type III (churning
contractions), isolated foci in lobules or secretory units throughout the gland contracted asynchronously and had very short
active states of about 1 min. By electron microscopy, myoepithelial cells could first be demonstrated in submandibular glands
developing either in vitro or in vivo, at 21 days postconception. Contractions in the cultured rudiments began as early as
18 days postconception. Since neither smooth nor striated muscle could be identified in these glands by electron microscopy,
the contractions are believed to result from myoepithelial activity that apparently may begin before ultrastructural evidence
of myoepithelial differentiation is contractile function and indirect evidence has lent ample support to this presumption,
the present study represents the first direct cinematographic demonstration and characterization of myoepithelial contractions,
under conditions in vitro. |
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Keywords: | myoepithelium contraction submandibular gland time-lapse photography organ culture |
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