Enzymes and cancer: Preparation and some properties of guanase from rabbit liver |
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| Authors: | R Currie F Bergel and R C Bray |
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| Institution: | Chester Beatty Research Institute, Institute of Cancer Research: Royal Cancer Hospital, London, S.W. 3 |
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| Abstract: | 1. Guanase has been purified 200-fold in 20% yield from the supernatant fraction of rabbit-liver homogenates, by using ammonium sulphate fractionation, calcium phosphate-gel adsorption and chromatography on DEAE-cellulose and Sephadex G-200. 2. Km with guanine as substrate at the optimum pH of 7·7 was found to be 1·05×10−5m. Q10 was 1·4 between 23° and 48°. 3. Substrate activity and pH optima of compounds related to guanine have been studied. 8-Azaguanine, 1-methylguanine, thioguanine and 1-methylthioguanine are all substrates. |
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