笃斯越桔耐弱碱突变体的离体筛选与鉴定

以笃斯越桔组培苗为试验材料,建立了EMS诱变体系,采用EMS诱变与NaHCO3共同胁迫处理的方法,筛选耐弱碱候选突变体,并进行了相关生理指标的鉴定,以明确其突变体株系的耐弱碱能力。结果显示:(1)0.4%EMS浸泡笃斯越桔茎段4h为较适宜的诱变剂量,存活率达46.7%;在含0.1%EMS的培养基中处理3d,存活率达50.0%;EMS浸泡处理后,NaHCO3适宜剂量为8mmol·L-1,经3次交替培养后,筛选出2株候选耐弱碱突变体。(2)相关生理指标鉴定表明,2株候选突变体的SOD和POD活性及游离脯氨酸含量显著高于对照,MDA含量显著低于对照。研究表明,该研究获得的笃斯越桔候选突变体具有一定的耐弱碱能力。

in vitro Screening and Identification of Mutants with Weak Alkali-tolerance of Vaccinium uliginosum Linn.

In this study,the tissue culture seedlings of Vaccinium uliginosum Linn.were used as material,the EMS mutation system has been established,the alkali-resistance mutants were screened by using EMS and NaHCO₃ combinative stress method,and identified with some physiological indexes.The results showed that:(1)0.4% EMS immersion the stem 4 h was the suitable mutation concentration,survival rate could reach 46.7%;when 0.1% EMS was applied into the medium 3 days,survival rate could reach 50%;the suitable concentration of NaHCO₃ was 8 mmol·L^-1,after three times alternating culture,two candidate mutants have been screened;(2)Identification of physiological index showed the activities of SOD,POD and proline content which significantly higher than those in control,the content of MDA which significantly lower than those in control.The study showed the mutants have some ability in weak alkali-tolerance.