Epstein-Barr Virus-Induced Expression of a Novel Human Vault RNA |
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Authors: | Constanze Nandy Jan Mrázek Heribert Stoiber Alexander Hüttenhofer |
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Institution: | 1 Innsbruck Biocenter, Division of Genomics and RNomics—Innsbruck Medical University, Fritz-Pregl-Strasse 3, 6020 Innsbruck, Austria 2 Department of Hygiene, Microbiology and Social Medicine, Section of Hygiene and Medical Microbiology, Innsbruck Medical University, Fritz-Pregl-Strasse 3, 6020 Innsbruck, Austria 3 Institut für Mikrobiologie und Hygiene, Abteilung Virologie, Haus 47, Universitätskliniken, D-66421 Homburg/Saar, Germany |
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Abstract: | Non-protein-coding RNAs (ncRNAs) have recently emerged on the scene of genomic research as prominent players in the regulation of gene expression. Many functionally characterized ncRNAs have been shown to be differentially expressed in various organisms during specific environmental or developmental conditions, thus establishing regulatory networks crucial for shaping cellular life. Here, we show that the expression of vault RNAs (vtRNAs) is specifically up-regulated in human lymphocytes upon infection by γ-herpesviruses, such as the Epstein-Barr virus and Kaposi's sarcoma virus. vtRNAs are ncRNAs that are integral to the vault complex, a gigantic (13 MDa) hollow ribonucleoprotein particle with a thus far elusive biological role. Stimulation of vtRNA expression by the Epstein-Barr virus was evident for all three canonical vtRNAs (hvg1-hvg3) and also for a novel ncRNA candidate, initially termed CBL-3. This ncRNA shares clear primary- and secondary-structure similarities with the three known vtRNAs. Importantly, CBL-3 co-sediments with intact vault particles in density gradients of various human cell lines, thus strongly indicating this ncRNA as a novel, fourth vault-complex-associated RNA. |
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Keywords: | vtRNA vault RNA EBV Epstein-Barr virus ncRNA non-protein-coding RNA EBER Epstein-Barr virus-encoded RNA miRNA microRNA CBL cord blood lymphocyte MVP major vault protein TEP1 telomerase-associated protein 1 CMV cytomegalovirus HHV human herpes virus Cox coxsackie B3 virus Parvo parvovirus strain G9 |
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