| 一株高效羽毛降解菌株的分离与鉴定 |
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| 引用本文： | 聂康康,姚大伟,郭俊清,王政,杨德吉.一株高效羽毛降解菌株的分离与鉴定[J].氨基酸和生物资源,2010,32(1):18-20. |
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| 作者姓名： | 聂康康 姚大伟 郭俊清 王政 杨德吉 |
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| 作者单位： | 南京农业大学动物医学院,江苏南京,210095;南京农业大学动物医学院,江苏南京,210095;南京农业大学动物医学院,江苏南京,210095;南京农业大学动物医学院,江苏南京,210095;南京农业大学动物医学院,江苏南京,210095 |
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| 摘 要： | 采用以羽毛粉为唯一碳源和氮源的培养基,从自然界中分离到一株能够高效降解羽毛角蛋白的细菌,经形态学观察,生理生化实验和16SrRNA基因鉴定,初步确定该菌株为短小芽孢杆菌(Bacillus pumilus),且命名为短小芽孢杆菌WHK4。发酵48 h时,羽毛粉降解率达到85.76%。本研究为微生物降解羽毛角蛋白提供了优良的菌株,在蛋白饲料生产中具有潜在的广泛的应用前景。
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| 关 键 词： | 羽毛 角蛋白 分离 鉴定 降解 |
Isolation and Identification of Keratin-Degrading Bacillus pumilus WHK4 |
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| NIE Kang-kang,YAO Da-wei,GUO Jun-qing,WANG Zheng,YANG De-ji.Isolation and Identification of Keratin-Degrading Bacillus pumilus WHK4[J].Amino Acids & Biotic Resources,2010,32(1):18-20. |
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| Authors: | NIE Kang-kang YAO Da-wei GUO Jun-qing WANG Zheng YANG De-ji |
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| Institution: | College of Veterinary Medicine;Nanjing Agriculture University;Nanjing 210095;China |
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| Abstract: | A strain of degrading feather keratin bacterium with high efficiency was isolated from environment by adopting the culture medium taking feather meal as sole carbon and nitrogen source.This strain was identified as Bacillus pumilus by morphology,physiological and biochemical experiments and 16SrDNA gene analysis,and named as the Bacillus pumilus WHK4.The degradation rate of feather keratin reached to 85.76% after 48h fermentation.The results indicated that WHK4 was an appropriate strain for exploring microo... |
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| Keywords: | feather keratin separation identification degradation |
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