Hyperthermia causes bovine mammary epithelial cell death by a mitochondrial-induced pathway |
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Authors: | Juan Du He-Shuang Di Liang Guo Zhong-Hao Li Gen-Lin Wang |
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Institution: | 1. Department of Animal Science and Technology, Naning Agricultural University, Nanjing 210095, China;2. Department of Animal Science, Anhui Technology College, Fengyang 233100, China |
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Abstract: | The effects of mild hyperthermia on bovine mammary epithelial cells exposed to 40 °C for 1 h were studied. The results showed that cell viability, ultrastructural features as well as mitochondrial function were significantly influenced by the mild heat treatment (40 °C). There was a considerate decrease in cell viability accompanied by cell loss resulting from apoptosis and necrosis followed by G2/M arrest. Cell death followed the typical cascade, namely decrease in the ratio of Bcl-2/Bax and mitochondrial membrane potential (ΔΨm), mitochondrial swelling and caspase-3 activities dramatically increased; DNA was also damaged. In conclusion, hyperthermia depresses cell viability and induces bovine mammary cell apoptosis and necrosis through the mitochondrial-triggered cell death pathway. |
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Keywords: | Mammary epithelial cells Apoptosis Necrosis Mitochondria |
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