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Production of ganoderic acid by Ganoderma lucidum RCKB-2010 and its therapeutic potential |
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| Authors: | Mohita Upadhyay Bhuvnesh Shrivastava Arti Jain Mazaahir Kidwai Sanjay Kumar James Gomes Dinesh G Goswami Amulya Kumar Panda Ramesh Chander Kuhad |
| Institution: | 1. Lignocellulose Biotechnology Laboratory, Department of Microbiology, University of Delhi South Campus, Biotech Park Building, Ground Floor, New Delhi, 110021, India 2. Green Research Laboratory, Department of Chemistry, University of Delhi, Delhi, 110007, India 3. School of Biological Sciences, Indian Institute of Technology Delhi, Hauz Khas, New Delhi, 110016, India 4. Product Development Cell, National Institute of Immunology, Aruna Asaf Ali Road, New Delhi, India, 110067 |
| Abstract: | The newly isolated basidiomycetous fungus, identified as Ganoderma lucidum RCKB-2010 was tested for production of ganoderic acid (GA) under submerged fermentation conditions. Production of GA under liquid static cultivation condition was found to be 2,755.88 mg L?1 on the 25th day of incubation, whereas under shaking cultivation conditions the maximum production of GA was observed to be 373.75 mg L?1. 1H NMR analysis revealed clearly that the fungal extracts possessed a lanostane skeleton, confirming the presence of GA. Interestingly, GA was found to have potential to inhibit the proliferation of HeLa cells and U87 human glioma cells in a dose dependent manner. In addition, GA was also found to possess antibacterial activity, exhibiting a minimal inhibitory concentration of 0.25 mg mL?1 against standard strains of Escherichia coli, Pseudomonas aeruginosa, Staphylococcus aureus and Staphylococcus epidermidis. GA produced in the present study holds potential as a potent anticancer agent. |
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