Statistical optimization of growth media for Paecilomyces lilacinus 6029 using non-edible oil cakes |
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Authors: | Abhishek Sharma Satyawati Sharma Aditya Mittal Satya Narayan Naik |
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Affiliation: | 1. Centre for Rural Development & Technology, Indian Institute of Technology Delhi, Hauz khas, New Delhi, 110016, India 2. Kusuma School of Biological Sciences, Indian Institute of Technology Delhi, Hauz khas, New Delhi, 110016, India
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Abstract: | The development of biofriendly and economical alternatives to chemical pesticides is a globally important scientific challenge. In this work, Karanja-based media conditions were optimized for obtaining high production of biomass and spores of a biocontrol agent, the entomopathogenic fungus Paecilomyces lilacinus 6029, using a two-step statistical approach coupled with rigorous experimentation. In the first step, non-edible Karanja cake was screened out as a major substrate from other oil cakes. In the second step, biomass production was maximized by applying response surface methodology to experimental variations in key physico-chemical factors: carbon/nitrogen (C/N) ratio and pH. This approach eventually predicted a maximum biomass production of 10.559 g/l with a medium having a C/N ratio of 35.88 and pH 5.9. An experimental production of 10.529 g/l biomass was obtained. The remarkable agreement between the predicted and the experimentally obtained biomass confirm the validity of the approach utilized to maximize production of P. lilacinus. |
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